Preparation and Fractionation of Rhizobium Bacteroids by Zone Sedimentation through Sucrose Gradients
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چکیده
منابع مشابه
Zonal Sedimentation Analysis on Sucrose Gradients.
Zonal sedimentation analysis on sucrose gradients allows estimation of the molecular size of an individual protein or a protein complex by centrifugation at a constant speed under nondenaturing conditions. This method is particularly suitable for globular proteins like the influenza A virus (IAV) protein hemagglutinin (HA). Here, I describe step by step a protocol used to evaluate the oligomeri...
متن کاملViability of Rhizobium bacteroids.
Bacteroids prepared from nodules of soybean and bean were tested for viability. Contrary to the prevailing view that bacteroids are nonviable, it was found that bacteroids averaged 90% viability, irrespective of Rhizobium strain, nodule age, or nodule environment.
متن کاملAmmonia assimilation by rhizobium cultures and bacteroids.
The enzymes involved in the assimilation of ammonia by free-living cultures of Rhizobium spp. are glutamine synthetase (EC. 6.o.I.2), glutamate synthase (L-glutamine:2-oxoglutarate amino transferase) and glutamate dehydrogenase (ED I.4.I.4). Under conditions of ammonia or nitrate limitation in a chemostat the assimilation of ammonia by cultures of R. leguminosarum, R. trifolii and R. japonicum ...
متن کاملCarbon Metabolism Enzymes of Rhizobium tropici Cultures and Bacteroids.
We determined the activities of selected enzymes involved in carbon metabolism in free-living cells of Rhizobium tropici CFN299 grown in minimal medium with different carbon sources and in bacteroids of the same strain. The set of enzymatic activities in sucrose-grown cells suggests that the pentose phosphate pathway, with the participation of the Entner-Doudoroff pathway, is probably the prima...
متن کاملOn the DNA Content of the Bacteroids of Rhizobium japonicum
DNA Content, Nodules, Bacteroids, Rhizobium, Soybean The DNA content of bacteroids from large nodules of soybean plants infected with Rhizobium japonicum strain 61 -A -101 was found to be 1.20 x 10~14 g per cell. Bacteroids from smaller nodules had slightly less DNA as did the stationary phase, free-living cells; both giving a value of 0.92 x 10-14 g per cell. In comparing these data with those...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1977
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.60.5.800